Techniques:

• Genome engineering (recombinase based homologous recombination)

• Golden Gate DNA Assembly

• Gibson DNA Assembly

• PCR

• RT-qPCR

• Genetic part development (i.e. constitutive and inducible promoters, antibiotic resistance markers, plasmid backbones, etc.)

• Targeted gene repression (CRISPR interference and antisense RNA)

• Flow cytometry

• Gel electrophoresis

• Fluorescence-based cell assays

• Bacteriophage amplification and engineering

• CRISPR editing (Cas12a/Cpfi, Cas9, Cas13)